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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
09/10/2013 |
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Data da última atualização: |
09/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, A. A.; ARAGÃO, C. W. S.; CASTRO, M. E. B. de; OLIVEIRA, J. V. de C.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M. |
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Afiliação: |
ANABELE AZEVEDO LIMA, UNB; CLARA WANDENKOLCK SILVA ARAGÃO, UNB; MARIA ELITA BATISTA DE CASTRO, CENARGEN; JULIANA VELASCO DE CASTRO OLIVEIRA, USP; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN MORAIS RIBEIRO, UNB. |
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Título: |
A recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
PLOSone, v. 8, n. 9, e74592, Sept. 2013. |
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DOI: |
10.1371/journal.pone.0074592 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest. MenosOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed hi... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/90688/1/journal.pone.0074592.pdf
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Marc: |
LEADER 02600naa a2200205 a 4500
001 1976569
005 2023-03-09
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0074592$2DOI
100 1 $aLIMA, A. A.
245 $aA recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity.$h[electronic resource]
260 $c2013
520 $aOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest.
650 $aSoja
700 1 $aARAGÃO, C. W. S.
700 1 $aCASTRO, M. E. B. de
700 1 $aOLIVEIRA, J. V. de C.
700 1 $aSOSA-GÓMEZ, D. R.
700 1 $aRIBEIRO, B. M.
773 $tPLOSone$gv. 8, n. 9, e74592, Sept. 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Origem |
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Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Cerrados; Embrapa Meio-Norte; Embrapa Milho e Sorgo; Embrapa Rondônia. |
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Data corrente: |
09/02/2011 |
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Data da última atualização: |
09/02/2011 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
GUIMARAES, L. J. M.; PACHECO, C. A. P.; GUIMARAES, P. E. O.; MEIRELLES, W. F.; PARENTONI, S. N.; SILVA, A. R.; COSTA, R. V. da; CRUZ, J. C.; PRADO, C. E. L.; MACHADO, A. T.; SOUZA, F. R. S. de; CECCON, G.; GODINHO, V. de P. C.; CARDOSO, M. J.; CARVALHO, H. W. L. de; GONCALVES, J. R. P.; VILARINHO, A. A.; ARCE, H.; VALENTINI, L.; DENUCCI, S.; SOUZA, J. C. de; MIRANDA, G. V.; ARNHOLD, E. |
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Afiliação: |
LAURO JOSE MOREIRA GUIMARAES, CNPMS; CLESO ANTONIO PATTO PACHECO, CNPMS; PAULO EVARISTO DE O GUIMARAES, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; SIDNEY NETTO PARENTONI, CNPMS; ADELMO RESENDE DA SILVA, CNPMS; RODRIGO VERAS DA COSTA, CNPMS; JOSE CARLOS CRUZ, CNPMS; CARLOS EDUARDO DO PRADO LEITE, CNPMS; ALTAIR TOLEDO MACHADO, CPAC; FRANCISCO RONALDO SARMANHO DE SOUZA, CPATU; GESSI CECCON, CPAO; VICENTE DE PAULO CAMPOS GODINHO, CPAF-RO; MILTON JOSE CARDOSO, CPAMN; HELIO WILSON LEMOS DE CARVALHO, CPATC; JOSE RICARDO PUPO GONCALVES, CPAA; ALOISIO ALCANTARA VILARINHO, CPAF-RR; HERCULES ARCE, Agraer-MS; LUCIA VALENTINI, Pesagro; SYLMAR DENUCCI, CATI; JOAO CANDIDO DE SOUZA, UFLA; GLAUCO VIEIRA MIRANDA, UFV; EMMANUEL ARNHOLD, UFG. |
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Título: |
Desempenho de variedades de milho: ano agrícola 2008/09. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2010. |
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Páginas: |
18 p. |
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Série: |
(Embrapa Milho e Sorgo. Comunicado técnico, 182). |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve por objetivo avaliar a produtividade de grãos, a adaptabilidade e estabilidade de produção, bem como outras características agronômicas, de 21 variedades de milho, dois híbridos intervarietais e dois híbridos duplos, no ano agrícola de 2008/09, em 30 localidades distribuídas nas regiões Sudeste, Centro- Oeste, Norte e Nordeste do Brasil. |
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Palavras-Chave: |
Adaptabilidade; Característica agronômica; Estabilidade. |
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Thesagro: |
Características Agronômicas; Grão; Produtividade; Variedade; Zea mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26808/1/CT-182.pdf
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Marc: |
LEADER 01715nam a2200493 a 4500
001 1876500
005 2011-02-09
008 2010 bl uuuu u0uu1 u #d
100 1 $aGUIMARAES, L. J. M.
245 $aDesempenho de variedades de milho$bano agrícola 2008/09.
260 $aSete Lagoas: Embrapa Milho e Sorgo$c2010
300 $a18 p.
490 $a(Embrapa Milho e Sorgo. Comunicado técnico, 182).
520 $aEste trabalho teve por objetivo avaliar a produtividade de grãos, a adaptabilidade e estabilidade de produção, bem como outras características agronômicas, de 21 variedades de milho, dois híbridos intervarietais e dois híbridos duplos, no ano agrícola de 2008/09, em 30 localidades distribuídas nas regiões Sudeste, Centro- Oeste, Norte e Nordeste do Brasil.
650 $aCaracterísticas Agronômicas
650 $aGrão
650 $aProdutividade
650 $aVariedade
650 $aZea mays
653 $aAdaptabilidade
653 $aCaracterística agronômica
653 $aEstabilidade
700 1 $aPACHECO, C. A. P.
700 1 $aGUIMARAES, P. E. O.
700 1 $aMEIRELLES, W. F.
700 1 $aPARENTONI, S. N.
700 1 $aSILVA, A. R.
700 1 $aCOSTA, R. V. da
700 1 $aCRUZ, J. C.
700 1 $aPRADO, C. E. L.
700 1 $aMACHADO, A. T.
700 1 $aSOUZA, F. R. S. de
700 1 $aCECCON, G.
700 1 $aGODINHO, V. de P. C.
700 1 $aCARDOSO, M. J.
700 1 $aCARVALHO, H. W. L. de
700 1 $aGONCALVES, J. R. P.
700 1 $aVILARINHO, A. A.
700 1 $aARCE, H.
700 1 $aVALENTINI, L.
700 1 $aDENUCCI, S.
700 1 $aSOUZA, J. C. de
700 1 $aMIRANDA, G. V.
700 1 $aARNHOLD, E.
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Embrapa Milho e Sorgo (CNPMS) |
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